e coli atcc 25 922 reference strain  (ATCC)

Journal: World Journal of Microbiology & Biotechnology

Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health

doi: 10.1007/s11274-026-05319-9

Figure Lengend Snippet: Distribution of the number of screened resistance genes per E. coli -like enterobacterial isolate from anaerobic digester influent and biofertilizer

Article Snippet: All isolates and the E. coli ATCC 25,922 reference strain yielded 6-mm measurements with lactic acid, indicating that the tested LA condition was non-discriminatory.

Techniques:

Journal: World Journal of Microbiology & Biotechnology

Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health

doi: 10.1007/s11274-026-05319-9

Figure Lengend Snippet: Absolute frequency of selected resistance-gene detection in E. coli -like enterobacterial isolates from anaerobic digester influent and biofertilizer

Article Snippet: All isolates and the E. coli ATCC 25,922 reference strain yielded 6-mm measurements with lactic acid, indicating that the tested LA condition was non-discriminatory.

Techniques:

Journal: World Journal of Microbiology & Biotechnology

Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health

doi: 10.1007/s11274-026-05319-9

Figure Lengend Snippet: Antimicrobial susceptibility profiles of E. coli -like enterobacterial isolates from anaerobic digester influent and biofertilizer. Legend: GEN (Gentamicin), AMC (Amoxicillin-clavulanate), CLO (Chloramphenicol), CAZ (Ceftazidime), MER (Meropenem), LVX (Levofloxacin), TET (Tetracycline). Colors represent CLSI susceptible, intermediate, and resistant classifications

Article Snippet: All isolates and the E. coli ATCC 25,922 reference strain yielded 6-mm measurements with lactic acid, indicating that the tested LA condition was non-discriminatory.

Techniques:

Journal: World Journal of Microbiology & Biotechnology

Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health

doi: 10.1007/s11274-026-05319-9

Figure Lengend Snippet: Distribution of the number of tested antimicrobial agents classified as resistant (R) per presumptive E. coli -like enterobacterial isolate from effluent and biofertilizer

Article Snippet: All isolates and the E. coli ATCC 25,922 reference strain yielded 6-mm measurements with lactic acid, indicating that the tested LA condition was non-discriminatory.

Techniques:

Journal: World Journal of Microbiology & Biotechnology

Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health

doi: 10.1007/s11274-026-05319-9

Figure Lengend Snippet: Biocide inhibition-zone profiles of E. coli -like enterobacterial isolates from effluent and biofertilizer. Data are presented as mean ± SD. The dashed line indicates the 6-mm disk diameter. H 2 O 2 inhibition zones differed significantly between matrices after Benjamini–Hochberg correction (q = 0.00493). LA 2% (2% Lactic Acid); BAC 0.28% (0.28% Benzalkonium chloride), H 2 O 2 3% (3% hydrogen peroxide); CHX 1% (1% chlorhexidine digluconate)

Article Snippet: All isolates and the E. coli ATCC 25,922 reference strain yielded 6-mm measurements with lactic acid, indicating that the tested LA condition was non-discriminatory.

Techniques: Inhibition

Journal: The Journal of Biological Chemistry

Article Title: Molecular basis of collagen triple helix recognition by VWF A-like domain two of collagen VII: Implications for interlaced anchoring fibril formation

doi: 10.1016/j.jbc.2026.113448

Figure Lengend Snippet: Binding of von Willebrand factor A3-like domains to bead-conjugated peptides. A , guest sequences of the peptides used for peptide-conjugated beads. Overlapping α1(III) 578 to 580 sequences of Pep 4 and Pep 5 are underlined. B , Peptide-conjugated beads were incubated with E. coli lysates expressing GST fusion proteins of collagen VII A2, VWF A3, or integrin α2I domains. Beads-bound proteins were separated by 12% SDS-PAGE under a reducing condition and visualized by Coomassie Brilliant Blue (CBB) staining. The molecular sizes of collagen VII A2, VWF A3, and integrin α2I are approximately 48 kDa. GST-accept resin and mock-coupled beads served as positive and negative controls, respectively. Pep 5 and Pep 6 were included as reference peptides for VWF A3 and integrin α2I binding.

Article Snippet: The resulting plasmid was verified by sequencing and transformed into SHuffle T7 Express Competent E. coli cells (New England Biolabs) for protein expression.

Techniques: Binding Assay, Incubation, Expressing, SDS Page, Staining

Journal: Cell

Article Title: Mitochondrial proteome of Acanthamoeba delineates aerobic and anaerobic pathways dynamically regulated by oxygen

doi: 10.1016/j.cell.2026.08.056

Figure Lengend Snippet: (A) Venn diagram of AcMitoCarta and the mitoproteome of Gawryluk et al. Heat maps show Z-scores for our PCP proteomics. (B) Box plots of DeepLoc2.1 mito scores for the sets of proteins depicted in panel A. Center lines, medians; box limits, 25th & 75th percentiles; whiskers, 1.5x interquartile range (IQR); dots, outliers. n = 316, 806, 197. (C) For the protein sets depicted in panel A, box plots of whole cell protein abundance (log 10 normalized MS intensity in PCP proteomics). Center lines, medians; box limits, 25th & 75th percentiles; whiskers, 1.5x IQR; dots, outliers. n = 301, 792, 153 sample points. (D) Comparison of mitochondrial protein homology relationships to those of all proteins from humans, yeast ( S. cerevisiae ), and Acanthamoeba (based on orthogroup membership; lineage indicates Opisthokonta or Amoebozoa). (E) Pie charts indicate shared AcMitoCarta orthogroup memberships with mitochondrial proteins of humans or yeast ( S. cerevisiae ). The fractions of unique mitochondrial proteins without a Pfam or without a bidirectional best hit (BBH) in Dictyostelium are also shown. (F) Pie charts as above, but for only the 316 AcMitoCarta-specific proteins indicated in the Venn diagram in panel A. (G) Presence and absence matrix for AcMitoCarta proteins and their orthologs across 203 eukaryotes, 34 prokaryotic clades, and a small set of archaea (rows indicate orthogroups in AcMitoCarta, columns indicate species, and blue marks indicate shared orthogroup membership). On the right, examples of orthogroups mapping to human MitoPathways, with tick marks indicating pathway members. (H) Human-centric MitoPathways analysis, with bar graphs indicating Acanthamoeba orthogroup mapping (or lack thereof) to mammalian MitoPathway members in the indicated pathways. (I) Fractions of AcMitoCarta proteins arising from prokaryotic HGT at the indicated evolutionary branch. (J) Acanthamoeba HupA protein domain schematic indicating a DNA binding domain shared with E. coli HupA (green). (K) Electromobility shift assay for DNA binding of WT & R66E mutant recombinant purified AcHupA. Yellow marks show baseline migration of DNA, which shifts when bound to HupA. The green triangle marks the lane in which a full shift above the baseline is observed. (L) Confocal microscopy of HupA-3xHA in Acanthamoeba showing co-localization with mtDNA. The signal intensity across one mitochondrion is quantified in the indicated box (yellow) as an example. See also .

Article Snippet: NiCo21(DE3) Competent E. coli , NEB , C2529H.

Techniques: Quantitative Proteomics, Comparison, Yeast, Analysis, Binding Assay, Electromobility Shift Assay, Mutagenesis, Recombinant, Purification, Migration, Confocal, Microscopy

Journal: Cell

Article Title: Mitochondrial proteome of Acanthamoeba delineates aerobic and anaerobic pathways dynamically regulated by oxygen

doi: 10.1016/j.cell.2026.08.056

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: NiCo21(DE3) Competent E. coli , NEB , C2529H.

Techniques: Produced, Cell-Signaling, Recombinant, Purification, Mutagenesis, Blocking Assay, Glass, Staining, Protease Inhibitor, Cation-Exchange Chromatography, Extraction, Sequencing, Modification, Mass Spectrometry, Bicinchoninic Acid Protein Assay, Cell Viability Assay, Profiling, Blue Native PAGE, Immunoprecipitation, Transcriptomics, Confocal, Microscopy, Plasmid Preparation, Software, Isolation, Sample Prep